SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

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	Trop. Plant Pathol. (1)
	PAB (1)

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	Gonsalves,Dennis (2)
	Nickel,Osmar (1)
	Souza Júnior,Manoel T. (1)
	Souza Júnior,Manoel Teixeira (1)

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Info:eu-repo/semantics/article (2)

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2005 (2)

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Brazil (2)

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Development of virus resistant transgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus

Trop. Plant Pathol.

Souza Júnior,Manoel T.; Nickel,Osmar; Gonsalves,Dennis.

Translatable and nontranslatable versions of the coat protein (cp) gene of a Papaya ringspot virus (PRSV) isolate collected in the state of Bahia, Brazil, were engineered for expression in Sunrise and Sunset Solo varieties of papaya (Carica papaya). The biolistic system was used to transform secondary somatic embryo cultures derived from immature zygotic embryos. Fifty-four transgenic lines, 26 translatable and 28 nontranslatable gene versions, were regenerated, with a transformation efficiency of 2.7%. Inoculation of cloned R0 plants with PRSV BR, PRSV HA or PRSV TH, Brazilian, Hawaiian and Thai isolates, respectively, revealed lines with mono-, double-, and triple-resistance. After molecular analysis and a preliminary agronomic evaluation, 13 R1 and R2...

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Carica papaya; Potyvirus; Resistance; Breeding; Virus.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400004

PAB

Souza Júnior,Manoel Teixeira; Gonsalves,Dennis.

The Papaya ringspot virus (PRSV) coat protein transgene present in 'Rainbow' and 'SunUp' papayas disclose high sequence similarity (>89%) to the cp gene from PRSV BR and TH. Despite this, both isolates are able to break down the resistance in 'Rainbow', while only the latter is able to do so in 'SunUp'. The objective of this work was to evaluate the degree of sequence similarity between the cp gene in the challenge isolate and the cp transgene in transgenic papayas resistant to PRSV. The production of a hybrid virus containing the genome backbone of PRSV HA up to the Apa I site in the NIb gene, and downstream from there, the sequence of PRSV TH was undertaken. This hybrid virus, PRSV HA/TH, was obtained and used to challenge 'Rainbow', 'SunUp', and an...